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GFP + ECs (around 9000 cells) were complemented with GFP ECs to reach a total of 40,000 cells and further processed for scRNA-seq library preparation and sequencing as described for total intestinal and mesenteric cells

Alternative repletion strategies have focused on oral administration of GSH precursors (e.g., cysteine and glycine supplementation 9 ), and intravenous administration of GSH, 10 which although promising, is invasive and inconvenient, and therefore unlikely to be a practical solution

Conflict of interest Author YT is a postdoctoral fellow at the joint workstation of Jiangsu University and Beijing Jingyi Group Co., Ltd

A large body of evidence indicates that various cellular metabolic pathways such as amino acid, lipid, glucose, and iron metabolism, can promote ferroptosis